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Abstract

Background: This training explored the possibility of circulating miR-21 as a biomarker for celiac illness (CD) in pediatric patients. The demographic and clinical characteristics were compared between groups. A case-control design was employed with 45 pediatric CD patients and 45 healthy controls. Aim: The present training stayed directed to exploring the expression of circulating miRNAs in those sick with celiac disease—three miRNAs—miR-21-3p. Methods: Blood samples were collected from participants, and RNA was isolated. miR-21 expression was quantified using STEM-LOOP RT-qPCR. Demographic data (age, gender, residence) and clinical features (family history, disease duration, symptoms) were collected. Serological tests for CD diagnosis were performed. Results: No important variances in age or gender were detected among groups. Family history of autoimmune diseases (diabetes and thyroid) was more common in CD patients. All celiac sick were positive for anti-tTG and anti-IgA antibodies. Common symptoms included diarrhea, anemia, abdominal pain, and vomiting. miR-21 expression was considerably higher in CD sick paralleled to controls. ROC investigation exposed great analytical accuracy for miR-21, with an AUC of 0.880 and a cut-off value of >4.08 for distinguishing CD from healthy controls. Notably, miR-21 expression stayed considerably lower in treated CD sick paralleled to untreated sick. Conclusion: The miRNA-21p3 can be used as good diagnostic biomarkers for CD.

Article Type

Article

First Page

9

Last Page

14

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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