Phenotypic detection of extended –spectrum betalactamase production in Proteus. mirabilis isolation from Patients with Significant Bacteriuria in Najaf provina

Abstract

250 mid-stream urine samples were collected from patients suspected of urinary tract infection (UTI) who were attended to Three hospitals (Medical Al-Sader City, Al-Hakeem, and Al-Zahra Maternity and Children) in Najaf provina during the period of October 2011 to January 2012 and screened for the presence of Proteus mirablis .The growth of ≥105 colony forming units/ml was considered as significant bacteriuria. A total of 198 (79.2%) significant bacteriuria were detected. The study showed higher incidence of UTI in females 129 (65.2%)than males 69 (34.8% )However , 52 (26.3%) isolates of Proteus mirabilis , 146 (73.7%) isolates other species pathogens. The 52 isolates of Proteus mirabilis were tested for their antibiotic resistance against 11 antibiotics by the Kirby-Bauer disk diffusion test. All the isolates were found to be resistant to at least 5 antibiotics to which they were subjected. Therefore, all these isolates were considered to be multidrug resistant. The majority 39 (75.0%) isolates of Proteus mirabilis were able to produce β-lactamase enzymes with rapid iodometric method. All isolates of Proteus mirabilis were tested for their ability to produce Extended-spectrum β-lactamase . The result showed that 18 (34.6%) isolates were able to produce ESBLs.