Morphological and Molecular Identification of Hospital Pseudomonas spp

Abstract

The study was designed for isolation and identification of Pseudomonas spp. from neonatal nosocomial infections, from Three Teaching Hospitals of Maternity and Pediatrics in Al-Mosul city. The total collected samples were 288,which included clinical(134samples collected from preterm and full-term neonates who less one year of age ) and environmental samples(154)from
 anuary 2011 till end July 2011, in order to evaluate the classical laboratory diagnostic procedures for diagnosis of Pseudomonas isolates and compared them with molecular technique on the basis Polymerase Chain Reaction (PCR). Pseudomonas spp. has been isolated and identified by using cultural method, biochemical tests and Mini API 20NE system as confirmative test. On the other hand, PCR technique was utilized to detected 16SrRNA gene as a house keeping gene, specific for diagnosis of Pseudomonas spp. The present study showed that the isolation percent of Pseudomonas spp. from clinical samples was 17/30 (18.8%)and 30/111(27%) from environmental samples, whereas the PCR results showed that there were high specificity (100%) in detection of Pseudomonas spp. versus each of cultural, biochemical and Mini API 20NE system