Circulating microRNA-182 overexpression as a biomarker for breast cancer

Abstract

Background:- MiR-182 is one of the most frequently studied cancer-related gene miRs and plays a crucial role in tumorigenesis , progression and may become a potential therapeutic target and biomarker of tumor diagnosis and prognosis.
Aim of study:-Estimation of miR-182 gene expression levels in both fresh tissues and serum of same breast cancer patients by using stem-loop follow by Taq-Man real time PCR (RT-PCR) technique and correlate the miR-182 gene expression with ER,PR and Her-2 by IHC technique .
Material and methods:- Stem-loop RT-PCR was performed to identify the level of miR-182 gene expression in both fresh tissues and serum of same breast cancer patients . The expression levels of miR-182 relative to mRNA of GAPDH were determined using the livak method . IHC were done for ER,PR and HER-2 .
Results:- Mean fold change of miR-182 was statistical significantly higher in breast cancer from paraneoplastic tissues , mean fold change of miR-182 was statistical significantly higher in serum of patients from apparently healthy control and miR‑182 serum level of patients with ER,PR positive was statistical significantly lower compared with the negative patients.
Conclusion:- The miR‑182 as a new original diagnostic and prognostic biomarker for breast cancer .