In order to study the prevalence of secondary infection in cutaneous Leishmaniasis and its relationship to the healing process , 76 Leishmaniotic patients were evaluated by three methods . The percentage of positive cases by injection with normal saline, part of skin biopsy and blood drops from lesion was 39 (68.4 %), 48 (84.2%) and 37 (65%) respectively while the percentage of nagative cases was 18 (31.6%), 9 (15.8%) and 20 (36%) respectevely . Secondary infection only were present in 57 (75%) patients, secondary bacterial infection were found in 52/ 57 (91%) patients , Secondary Fungal infection was found in 5/57 (9%) patients , gram positive bacteria only were present in 48/52 (92%) , gram negetive bacteria were present in 4 /52 (8%) patients. The secondary infection gram positive bacteria which appear Staphylococcus aureus 45/48 (93.7%) ,Streptococcus pyogenes 2/48 (4.2%) ,Staphylococcus epidermidis 1/48 (2.1%) , While gram negative bacteria appear Escherichia coli 2/4 (50%) ,Pseudomonas aeruginosa 1/4 (25%), klebsiella spp. 1/4 (25%) .