The seed of trigonella Feneum - graecum was extracted with 70% ethanol, the antibacterial activity of different concentration of the extract were tested to inhibition of some Gram positive pathogenicc bacteria such as Staphylococcus aureus. Diplcoccus pneumonia, Staphylococcus epidermidis, Streptococcus facials and Gram negative such as Escherichia coli, Klebsiella spp & Pseudomonas aeroginousa. The high concentration ≥2mg/ml showed antibacterial activity against gram-positive bacteria, while pseudomonas auregenosa was resistant to all concentration of extract. The results indicated that the extract of fenugreek plant seeds is important in treatment of bacterial infection & drugs industries.