This study was carried out in Al-Habobi Hospital for Children and Maternity in Al-Nasseria city. Fifty-four blood cultures were obtained from 54 children (age from 7 days to three years) with history of febrile, focal infection and history of illness. Forty-three isolates were isolated from forty patients (22 males and 18 females) with blood steam infections. Gram positive microorganisms were predominate (60.46%)such as Streptococcus pneumoniae (18.60%),Enterococcus faecalis, Staphylococcus aureus (16.22%),Coaglase negative Staphylococci (9.30%), and Gram negative bacteria, Escherichia coli (16.22%), Pseudomonas spp. ,Hemophilus influenza (4.65%) respectively. Results of antimicrobial sensitivity which showed that Ceftriaxon, Cefotaxime, Augmentin, Amoxicillin and Ampiclox were the high effective against Gram positive bacteria, and the Gentamycin, Amikacin and Trimotheprim the most suitable antimicrobial against Gram negative bacteria.
