Identification of 5S rRNA gene of Enterobius vermicularis

Abstract

Background:   The present study was carried out from October 2012  to the end of  September  2013. The study included a collection of  two hundred -twenty four stool sample from deferent ages,  Ages were vary from one to sixty years old for detection Enterobius vermicularis .Stool samples were used direct wet mount ,concentration method and stained with iodine (( the eggs ,larvae and adult )) had been noticed by microscop , 10x ,40x and oil immersion lens (100x). The results showed that 146 (65%)   out of 224 were microscopically positive. The stool samples were store in -20°C until use. In molecular study of this parasite PCR technique was conducted, to detect one gene 5sRNA by using specific primers for Enterobius vermicularis, the results showed that 126(56.3%)  samples out of 224 were positive. In comparison between microscopic and PCR methods the results showed that 146 (65%), 126(56.3%) out of 224 were positive respectively,with important of PCR technique in diagnosis occult enterobiasis 30(23%).         Materials and methods: stool samples were used in this study to detect the presence one specific 5SrRNA gene . 224 randomly stool sample were determined by the polymerase chain reaction method. Results: The 5SrRNA gene may be facilities in diagnosis occult and non- occult Enterobiasis .Conclusion: Polymerase Chain Reaction technique was useful for identification of Enetrobius Vermicularis and occult cases.This study aimed to role of Polymerase chain reaction(PCR) technique used to prove the DNA of Enerobius vermicularis.