Detection of mec A gene in Staphylococcus aureus from Osteomyelitis infections

Abstract

Background: Staphylococcus aureus is a major pathogen that associated with serious community acquired and nosocomial diseases. In view of the present problem the study aimed to isolation of S. aureus within osteomyelitis samples and amplified the mecA gene of some antibiotic resistant isolates.
Methodology: S. aureus were isolated from osteomyelitis and all isolates screened for antibiotic susceptibility test to detection of mecA gene.
Results: 29 swabs were taken from both sex patients related to Al- Sammawa Teaching hospital at period from September 2013 to march 2014. Only 14(48.27%) patients had S. aureus and 19 isolates yielded from all samples. These isolates showed pronounced resistance for a group of active antibiotics. Five isolates have full (100%) resistance to oxacillin, penicillin and cefoxitin so considered as Methicillin Resistant Staphylococcus aureus (MRSA). Genetic identification showed that five S. aureus isolates have mec A gene in their genome.
Conclusion: This study highlights the presence osteomyelitis infection in both sex with little incidence. S. aureus one of the important pathogens that have resistance to many antibiotics due to mecA gene.