Cultivation of Entamoeba species in Lock’s -egg medium and differentiation of E.moshkoviskii ,E.dispar and Ehistolytica by using single round polymerase chain reaction

Abstract

The genus Entamoeba contains many species, three of which are  identical (Entamoeba histolytica, Entamoeba dispar and  Entamoeba moshkovskii ) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and mortality worldwide in humans. Although recent studies highlight the recovery of E. dispar andE. moshkovskii from patients with gastrointestinal symptoms. The  cultivation  of  three identical   Entamoeba  species   was  done  successfully  in   Lock’s-egg medium  , a high  number  of  trophozoite   were  found   in  culture .these  three identical Entamoeba  species did  not  differentiation by  using  microscope .but  the  devolution of  scientific  they  could  differentiation  by  using  PCR  technique when  extraction the  DNA in  stool  samples by  using  specific bands  and  primers