The role of Laminin111 and Matrix metalloproteinase-2 Immunohistochemistry Expression in Prediction of Laryngeal Squamous Cell Carcinoma Prognosis

Abstract

Objective: To investigate the role of laminin111 (LAM111) and matrix metalloproteinase-2 (MMP2) as a prognostic predictors in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: The study was carried out on 30 patients with LSCC treated at the ENT Department, Al-Diwaniya Hospital in Al-Qadisiya province- Iraq. The clinical and histological data consisted of sex, age, site, clinical presentation, stage of the tumor, lymph node (LN) metastasis, and immunohistochemical studies (IHC). The IHC study was performed using anti LAM111and anti MMP2 monoclonal antibodies. Results: The majority of patients were above 50 years (73.33%), male affected more than the female with male to female ratio 2.8:1, 50% of tumors were located in glottic region, (86.67%) presented as mass. Most of the tumors were classified as well differentiated LSCC (53.33%), (76.67%) were recorded as negative LN metastasis, and 14 (46.67%) patients were in disease stage II. LAM111showed significant positive correlation with LN metastasis (P=0.027) and tumor stage (P=0.018), while MMP2 revealed significant positive correlation only with LN metastasis (P=0.029). There was a significant positive correlation between LAM111and MMP2 (P=0.014). Conclusion: Both LAM111 and MMP2 are poor prognostic markers for LSCC and predict LN metastasis.