Distribution of Granulicatella adiacens and Porphyromonas gingivalis among ortho and non-orthodontic Patients with Gingivitis in Kufa City /Iraq.

Abstract

This study aimed to investigate the distribution of Granulicatella adiacens and Porphyromonas gingivalis and the role of orthodontic wire on antibiotic resistance pattern of bacterial isolates. A total of 78 gingival swab samples have been collected from patient with orthodontic wires suffering from gingivitis and 71 samples were collected from healthy without orthodontic wire during four months from private dental clinics.
The results of isolation and identification of bacterial isolates by using culture and conventional biochemical tests as well as molecular technique using 16S rRNA for detection of G. adiacens and insertion seqence element IS1126 for detection of P. gingivalis showed that 54 (29.6%) isolates were belong to G adiacens and 4 isolates were belong to P gingivalis.
To explain the role of orthodontic wires on bacterial isolates a mutation experiment was carried out. The result showed the same change has been obtained when using both NiTi and stainless steel wire on G. adiacens and P. gingivalis after 24hr-96hr of incubation. Antibiotic resistance pattern of both original and treated isolates with NiTi and stainless steel showed increased in antibiotic resistance to bacitracin, ceftazidim, ogmentin, and erythromycin while other antibiotic remain sensitive such as cefotaxim and amikacin for all isolates.