Effect Of Amlodipine In Amelioration Of Global Cerebral I/R Injury In Rat

Abstract

Background: Cerebral ischemia–reperfusion injury (IRI) is a complex process resulting in cellular damage and death. Ischemia and reperfusion in the brain, as in other organs, induces an inflammatory response which may exacerbate initial levels of tissue injury.
Objectives: This study was undertaken to investigate the possible neuroprotective activity of amlodipine in amelioration of global cerebral I/R injury in rat model.
Materials and Methods: Adult sprague-dawley rats were randomized in to 4 groups as follow: group I, sham group, rats underwent the same anesthesia and surgical procedure as the control group with out bilateral common carotid artery occlusion (BCCAO) ; group 2 control group (induced-untreated), rats underwent 30 min of global cerebral ischemia via ( bilateral common carotid artery occlusion (BCCAO) followed by 1 hour of reperfusion ; group 3, Control – Vehicle, as control group but rats received daily for 10 days before the surgery the vehicle of amlodipine drug normal saline intraperitoneally (IP),the dose of vehicle was (0.9% Nacl) ,(1 ml/kg/day) ; group 4, amlodipine treated group, as control goup ,but rats received daily amlodipine intraperitoneally (IP), the dose of amlodipine was (10 mg/kg /day) for 10 days before the surgery .
Results: Compared with the sham group, levels of cerebral ICAM-1, increased significantly (p<0.05), amlodipine, significantly oppose the increase in cerebral level of ICAM-1 (P < 0.05). Histological analysis revealed that amlodipine markedly reduced (P < 0.05) the severity of brain injury in the rats underwent bilateral common carotid artery occlusion (BCCAO) .
Conclusions: The results of the present study revealed that pretreatment with amlodipine may ameliorate the global cerebral ischemia-reperfusion injury by anti-inflammatory effect.