Phylogenic study of toxoplasmosis in aborted women in Al- Diwnaniya province
DOI:
https://doi.org/10.28922/qmj.2020.16.1.10-15Keywords:
T.gondii, human, camel and goat, SAG3, NpcrAbstract
The current study included the detection of Toxoplasma gondii from aborted women attented to the Maternity and Childhood Teaching Hospital to detected the rate of abortion due to toxoplasmosis using PCR technique, the samples which was used placenta and fetus, this study also identify the differentiation between it by phylogenic with SAG3 gene.
A total of 60 ( placenta and fetus) samples were collected randomly from aborted women who received to the Maternity and Childhood Teaching Hospital.
The present study include molecular method to detected small subunit ribosomal RNA ( ssRNA) using Nested Polymerase Chain Reaction ( nPCR) ,the results of the nPCR for ssRNA gene, the result showed that the rate of toxoplasmosis in the aborted women was Positive 60%. (There is a statistically significant between species in P <0.05).
The present study also detected for Surface Antigen (SAG3) gene to conform the nPCP result and to send the samples for sequences.
The SAGA gene showed 100% positive results according to the ssRNA gene .
T.gondii isolates surface Ag SAG3 gene with NCBI BLAST Toxoplasma gondii isolates, and the DNA nucleotides sequencing analysis of surface Ag SAG3 complete gene was show clear gentic variation between isolates from different host.
