Histopathological study on maternal pancreas, placenta and fetal pancreas at 18th day postgestation of streptozotocin induced maternal diabetic rats (Rattus norvegicus), and the effect of n-butanolic extract of celery seeds (Apium graveolens)

Abstract

The study is designed to clarify the influence of induced maternal diabetes mellitus on placenta and the fetus at 18^th day of gestation. Diabetes mellitus was induced in (24) female's rats (Rattus norvegicus) before mating by streptozotocin (60 mg/kg of animal body weight) one dose intrapersonal injection, all animals were isolated and divided into two groups (non-diabetic and diabetic group), each group was composed of (12) female, also each group was subdivided into two groups, healthy females group as control and the subgroup treated with n-butanolic extract of celery seeds (60 mg/kg of body weight daily). The diabetic group also had been subdivided into two groups, another one of them had been treated with celery seeds extraction, and one had diabetes mellitus without treatment. There were many macroscopic observations reported by the current study,  include the still birth in addition to differences in size between the fetuses which was tend to increased (macrosomia) in the diabetic groups resulted from hyperglycemic mother. Microscopic study detected harmful effects of diabetes mellitus on the tissues, while in the other hand there are a regeneration in islets of Langerhans when treated the animals with n-butanolic extraction of celery seeds with slightly heal in certain points in tissues, also had seen  some of the side effects of treated with extract.