The local Propolis as protective and therapeutic modulator on some biochemical parameters in diabetic nephropathic rats

Abstract

The aim of the present study was to investigate the effect of daily oral administration of local Ethanolic Extract of Propolis (EPP) on blood glucose, antioxidant defense system and kidney function in streptozotocin (STZ)-induced diabetic rats to show the ameliorating and partly curative effects in STZ-induced rats(60mg/kg) intrapretonial (i.p). Seventy five rats (150-160gm) were divided into 5 groups, normal rats, diabetic rats, normal treated with local EEP for 6 weeks, pretreated with local EEP for 3 weeks then induced diabetic in rats for 3 weeks and the last group, after 3 weeks(normal) induced diabetic in rats then post- treated with local EEP for 3 weeks. The dose of EEP 200mg/kg for the last three groups, and groups 2, 4 and 5 were induced diabetes in the same time at the end of the first 3 weeks.
After 6 weeks , serum of samples were collected from anesthetic rats to determined the biochemical alterations.
Antioxidant status in rats were estimated by determined the Glutathione(GSH) concentration, and Glutathion peroxidase (GPx) activity, and also measured renal function by the tests of Serum Urea and Creatinine concentrations, the result of this research indicated the effect of local EEP as protective or therapeutic in this experiment, the diabetic group showed decreased significantly(p<0.05) in body weight gain, increased(p<0.05) blood glucose, decreased significantly(p<0.05) activity of GPx and GSH concentration and increased significantly(p<0.05) serum Urea and Creatinine concentrations, as well as local EEP was moderated and effected significantly decrease(p<0.05) glucose level, Urea and Creatinine concentrations, increased significantly(p<0.05) activity of GPx and GSH concentration, as a result, it led to protect the kidney from damage by moderated their functions.