the Investigating the Interplay Between IL-12 Expression and miRNA-140-5p Regulation in the Pathogenesis of Asthma: Implications for Novel Diagnostic and Therapeutic Approaches

المؤلفون

  • Hawraa Khalid Masters student Department of Microbiology, College of Medicine, University of Al-Qadisiyah
  • Manal Mohammed Kadham Department of Microbiology, College of Medicine, University of Al-Qadisiyah

الملخص

Background: Asthma involves chronic airway inflammation with immune dysregulation. IL-12 and miRNA-140-5p are emerging markers linked to this process.

Aim: To assess IL-12 levels and miRNA-140-5p expression in asthma patients versus healthy controls.

 Methods: The study included 60 asthma sick and 40 fit controls. Serum IL-12 ranks stayed measured using ELISA, and miRNA-140-5p expression was analyzed by RT-qPCR. Results:The results IL-12 ranks stayed considerably upper in sick (mean 1432.5 pg/mL) than controls (560.5 pg/mL; p = 0.001), with perfect diagnostic accuracy (AUC = 1.0). miRNA-140-5p expression was significantly lower in patients (fold change 1.46 vs. 10.11; P < 0.001).

Conclusion: Elevated IL-12 and reduced miRNA-140-5p may serve as simple, non-invasive biomarkers reflecting airway inflammation and alteration in asthma.

التنزيلات

منشور

2025-12-31

إصدار

مجلد 21 عدد 2 (2025)

القسم

Microbiology