Pneumococcal pneumonia is more common in the very young and the very old ages. Pneumococci are the most common cause of meningitis in adults. Especially at risk are alcoholics and people with chronic otitis, sinusitis, mastoiditis. We compared the relative efficacy of three methods for the isolation of Streptococcus pneumoniae microorganism in both pneumococcal meningitis and pneumococcal pneumonia in both lower respiratory secretion and cerebrospinal fluid depending on results from total speciemens collected (Cerebrospinal fluid and respiratory secretion). These 100 speciemens were subcultured on different three types of blood agar plates we found that Streptococcus pneumonia was isolated at a frequency of 70% with 5% sheep Blood Agar or 5% Sheep Blood Agar containing 5ug/ml of Gentamicin per milliliter, both incubated in 5% Co2. Anaerobic incubation of 5% sheep blood agar containing 5ug/ml of Gentamicin per milliliter enhanced the recovery rate of Streptococcus pneumonia to 90%. The differences in the recovery rates of Streptococcus pneumoniae isolated from both pneumococcal meningitis and pneumococcal pneumonia by various culture media and conditions were analyzed.
