This study aimed to show the effect of sweeteners Sodium cyclamate and aspartame on culture media and its role in the enhancement of the growth of some clinical isolates of Escherichia coli, Klebsiella pneumoniae and Enterobacter. Results showed that Klebsiella pneumoniae isolates were unable to grow in low concentration of aspartame (0.9, 1.8 mg/ml) but they showed well growth at relatively high concentrations of this compound. Results also showed that Enterobacter spp was the best growers (109 cells/ml) at high concentration of aspartame (9.0 mg/ml) P> 0.05 . Sodium cyclamate enhanced the growth of E.coli, Klebsiella pneumoniae ,and Enterobacter spp. at different concentrations P< 0.001. Escherichia coli showed heavy growth and was the best growers (109-1010 cells/ml) at different concentrations of Sodium cyclamate.
