Detection of Extended Spectrum β-lactamases Producing by Klebsiella pneumonia Isolated from Urinary tract Infection Patients by Using mPCR Technique

المؤلفون

  • Wafaa Abdelwahed Jaheel Al-Kaaby College of Biotechnology/ Al-Qadisiyah University

DOI:

https://doi.org/10.28922/qmj.2016.12.22.59-64

الكلمات المفتاحية:

K. pneumonia، β-Lactamase، antimicrobial.

الملخص

Objective: In this study Klebsiella pneumonia where isolated from urine samples and detection of extended spectrum β-Lactamases by using mPCR technique.
Method: This isolated subjected to the antimicrobial susceptibility test (Disc diffusion) for some antibiotics following by multiplex PCR techniques for detection extended spectrum β-Lactamase genes (bla TEM, bla SHV, bla CTX-M and bla AMPC).
Results: The extended spectrum β-Lactamase genes by PCR techniques were given bla CTX-M (30/93.75%), bla SHV (25/78.12%), bla TEM (18/56.25%) and bla AMPC (22/68.15%), respectively.
Conclusion: K. pneumonia isolates of urinary tract infection patients highly associated with the emergence of bla CTX-M β-Lactamase that provides useful good treatment.

التنزيلات

منشور

2017-09-25

إصدار

القسم

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