Chromosomal Aberration Study of Human Lymphocytes exposed to Americium -241 in vitro

الملخص

This study was intended to decide the impact of ?-partcales for acceptance of chromosome deviation. The venous blood sample was drawn from 30 years old healthy male donor. The blood sample after cultured in RPMI was divided into two groups, the first was as control and the second was exposed to radiation from Am-241 to detect the effect of it on chromosomal aberration (CA). The cultures exposed to Am-241 have dicentric (52.8%), breaks (20%) , fragments(18.5%) and (6.8%) ring chromosomes . There was significant differences (p ? 0.5 ) compared with control sample. Dicentric show high percentage of chromosomal aberration compared with other CA. The mean of mitotic index (MI) and stander deviation was (2.212±2.236) for radiation exposed sample higher than that of control was(1.565±1.581). It has been concluded the exposure to low ?-particales could caused chromosomal aberrations in human cells.