Pseudomonas aeruginosa، virulence factor، PCR، amino glycoside group
الملخص
Pseudomonas aeruginosa became an important cause of wound and burn infections and serious mortality in burn patients. The virulence factors of these bacteria including their capacity to form biofilm, their ability to hold antibiotics resistance genes as well as rapid motility in vivo and in vitro. Thus the present study was designed to highlight some the virulence factors among different strains of Pseudomonas aeruginosa. All isolated strains (10 isolates) had the capacity to produce biofilm ,one of them (No.3) has shown high level of biofilm (OD.2.5) and it was resisted to most antimicrobial agents; for this reason further investigation was carried out including gene expression by PCR technique and motility. Results showed that all strains were able to resist most common antibiotics; the molecular study for Strain 3 gave clear band of gene size 1.2pb which is responsible for resistance to aminoglycoside group. Understanding the virulence factors mechanisms of Pseudomonas aeruginosa is of a big importance in order to reduce the mortality in burn patients.
