The prognostic significance of XL DLEU/LAMP (13q14 deletion) in CCL patients: a cross section study

الملخص

Background: Many cytogenetic abnormalities were detected in CLL, one of them. Deletion of 13q14 region which is found in more than 50% of CLL patient. 13q deletion is the most common cytogenetic abnormality detected by fluorescence in situ hybridization (FISH) and has historically been associated with good prognosis. During the last years, several studies have revealed some insights in the candidate genes located at 13 q that could be responsible for CLL pathogenesis,as well as in the prognostic heterogeneity of 13q- deleted patients.

Aim of the study: evaluation of the prognostic value of 13q chromosome deletion in patients with CLL in correlation to complete blood picture, absolute lymphocyte count, prolymphocyte count and modified Rai staging.

Patients and method: This cross-sectional study was conducted on thirty adult with newly diagnosed and Denovo B-CLL patients tested for the expression of 13q deletion using Fluorescence Insitu Hybridization, from March 2018 to July 2018 and the diagnosis was document on the morphology and immunephenotyping of the peripheral blood sample using a four-color flow cytometer in the Nursing Home Hospital / flow cytometry department of the Medical City in Baghdad.

Results: XL DLEU/LAMP (13q14 deletion) was seen in 7 (23.3 %). 13q14 deletion was not significantly correlated to age, gender, splenomegaly, lymphadenopathy or hepatomegaly. There was highly significant difference in mean Hb between CLL patients with positive 13q14 deletion and those with no deletion (P = 0.001), being higher in patients with positive 13q14 deletion than those without deletion, 12.96 ± 1.41 g/dl versus 10.14 ±1.74 g/dl, respectively. In addition, all CLL patients with positive 13q14 deletion were free of anemia, whereas anemia (Hb < 11 g/dl) was seen in 17 (73.9 %) of patients with no deletion; the variation in 13q14 deletion according to anemia was highly significant (P = 0.001).

Conclusion: indirectly, one can suppose that chromosome 13q.14 deletion carry good prognosis in CLL patients; however, it appears better to link such deletion to survival rate in order to get better idea about its prognostic significance.