Isolation of Enterobacteraerogenes from urine of patient with urinary tracts infection and studying pathogenicity of Enterobacteraerogenes in mice

المؤلفون

  • Liath AbdulHassan Mohamed Biology Department/College of Science/Al-Muthanna University
  • Jawad Rafat AbdulHassan Mohamed Biology Department/College of Science/Al-Muthanna University
  • Jawad Ali Anok Najum Biology Department/College of Science/Al-Muthanna University

DOI:

https://doi.org/10.28922/qmj.2015.11.19.173-179

الملخص

The present study aimed to isolate Enterobacteraerogenes from urine of patient with UTI and showing its pathogenicity in mice, a total 150 urine specimens were collected and cultured on MacConky agare then tested biochemically and with API- 20 to confirm diagnosis of Enterobacter aerogenes, after that injected 4 groups of mice with (108,109 and 1010 CFU/ml) respectively, while fourth group consider as a control group, also extracted the cell wall from Enterobacteraerogenes and used four groups of mice to injected with different concentration(300, 200 and 150 μ/ml) of extracted cell wallrespectively, while fourth group consider as a control group. Results shows that 11 isolates of Enterobacter were obtained from urine and out of 11 isolates 8 isolates were belong to Enterobacter aerogenes. Bacterial isolation from internal organs showed very heavy isolation of Bactria in dose 1010 CFU/ml as compared with other dose, histopathological changes of organs for mice injected with live bacteria and extracted cell wall revealed same the tissue damage as compared with control groups.

التنزيلات

منشور

2017-07-24

إصدار

القسم

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