Gene and Alleles of TYK2 (rs11085727) and TYK2 (rs34536443) polymorphisms in COVID-19 Patients using ARMS-PCR.

الملخص

The clinical presentation of COVID-19 varies greatly from case to another, with host characteristics playing a pivotal role. The immune system is a pivotal function in establishing the course of SARS-COV-2 infection. Severe instances of COVID-19 and the resulting death are caused less by the virus itself than by the destructive unrestrained immune response. Among the host determinants for poor disease prognosis is probably genetic vulnerability to dysregulated immune response. The objective of this work is to examine some of the host genetic variables that are being studied in relation to the severity of COVID-19. The complex relationship between autoimmunity and the pathophysiology of COVID-19 was examined. Patients with COVID-19 were recruited from AL-Dewaniyah Teaching Hospital and AL-Hussain Teaching Hospital in Karbala city. All patients had been diagnosed with the virus using real-time polymerase chain reaction. A total of 105 blood samples (35 from the mild group, 35 from the severe group, and 35 from the controlled group) were obtained in EDTA tubes. Orderly to investigate the correlation between the progression of disease and variations in specific regions of host DNA (TYK2), DNA extraction, and genetic analysis (ARMS- PCR). The findings of the ARMS-PCR assay showed insignificant difference between severity of COVID-19 in the present study. Genotype distributions were shown both TYK2 (rs11085727) and TYK2 (rs34536443) polymorphisms. There was insignificant difference between the observed and expected distribution of healthy by TYK2 (rs11085727) and TYK2 (rs34536443) genotypes. According to the results of ARMS-PCR, there was insignificant difference between the severity and its mildness in the current study patients.