IL-13 serum levels along with its genetic polymorphism as a risk factor for chronic rhinosinusitis in Iraqi patients
IL-13 and CRS
Abstract
Background: Chronic rhinosinusitis (CRS) represents a prolonged inflammatory condition of both nasal cavity and paranasal sinuses which extends more than 12 weeks without any sign of improvement. This condition affects work attendance along with productivity levels and quality of life detrimentally.
Objective: To find out whether IL-13 rs1800925 C/T-1055 genetic polymorphism and its serum levels have a risk value for CRS disease.
Methods: Three groups have been used as the basis for a case-control study. Thirty patients with a history of chronic rhinosinusitis with nasal polyp; thirty patients with a history of chronic rhinosinusitis without nasal polyp; and thirty healthy volunteers with no family history of CRS were included in the third group as a control subject. blood samples were collected from each patients and control group. To investigate the genetic polymorphism for IL-13 rs1800925 C/T-1055, two milliliters of blood were collected in an EDTA tube for complete DNA extraction. Three milliliters of blood were collected in a gel tube, after which the serum was separated by centrifugation and used for the ELISA test to measure the amount of IL-13 in the serum.
Results: This study revealed no statistical differences between patients affected by CRS across different sex groups and age ranges. The CT genotype frequency of IL13 showed a significant increase in patients with nasal polyps than patients without nasal polyps (p = 0.007). Serum IL-13 levels were found to be significantly higher in the CRS group than in the control group, with the highest levels seen in the nasal polyps group (p=0.042).
Conclusion: present data indicate a relationship between the IL-13 rs1800925 C/T-1055 SNP and development of chronic rhinosinusitis in Iraqi patients. Because such genotype found to affect on IL-13 concentration in the serum of those patients, which make the condition more sever.
