Molecular and immunological study for detection of IL-6 in men infected with Trichomonas vaginalis parasite in Al-Najaf province; Iraq

Saleem k.AL- Hadraawy; Salh M. M. Al-Kafagy; Maysoon K. A .Al-Hadraawy

doi:10.28922/qmj.2014.10.18.144-148

Authors

Saleem k.AL- Hadraawy *Sciences of collage Kufa University
Salh M. M. Al-Kafagy Faculty of Medicine / Kufa university
Maysoon K. A .Al-Hadraawy Medical laboratory / Department of Technical institute

DOI:

https://doi.org/10.28922/qmj.2014.10.18.144-148

Abstract

The aim of this research was to diagnosis of Trichomonas vaginalis by use PCR technique and evaluate interleukin-6 (IL-6) in men infected with Trichomonas vaginalis; urine and blood specimens were collected from 85 men whom have visited the department of infertility at Al-Sadder medical city, Al-Zahra Hospital in Najaf Province during the period from June till October, 2012. Twenty healthy looking age matched men taken to serum tubes and serum was separated. Serum was used for evolution of the IL-6.The IL-6 was evaluated in serum using ELISA technique .Trichomonas vaginalis was isolated from 15 men with a prevalence rate 17.64% by using PCR technique. The results revealed a significant increase in IL-6 in men infected with Trichomonas vaginalis in compared to control group and the PCR is accurate method used in diagnosis of Trichomonas vaginalis parasite.