the Molecular detection of blaIMP and ompA genes in Acinetobacter baumannii isolation from burn wounds infaction
blaIMP , ompA and burn wounds infaction
Abstract
background : antibiotc resistance in Acinetobacter baumannii can be acquired rapidly due to chromosomal mutation, and its antibiotc- resistance producing Metallo-beta -lactamases( MBL) has been claimed to have great contribution to nosocomial infaction.
Aim of study : This purpose of this resea rch for detection blaIMP carbapenemase-production isolates utilizing PCR-taechinque.
Materials and Methods: A cross-sectional research was conducted among totally 150 clinical samples for patients within various ages-groupes as well as sexes- patients underwent testing throughout the current research between October 2024 until April 2025 after being taken into the Specialist Burns-Center inside Al-Diwaniyah . Every-patient had a burn swab-collected. The samples were brought to the research center within a sterilized leak-proof containers.every single one of these specimens went through cultivation on 5% blood-agar and MacConkey-agar . The following by incubating aerobically durin 24-hours at 37 C.
A.S.T was used to isolate-organisms that produced- carbapenemase that, following the conventional-PCR method had been utilized to identify the blaIMP as well as OMPa genes .
Results : The present cross-section study enrolled 150 swab samples from patients with burna were investigated for bacterial infaction using bacterological culture of swab samples . The isolation procedure resulted in obtaining of 30(20.0%) isolates of Gram positive, 94 (62.7%) of Gram negative bacteria and 26( 17.3%) of mixed growth . MDR and XDR rates among a. baumannii isolates were 43.2% and 56.7% , respectively. Genotypic analysis of A. baumannii revealed high frequencies of ompA (51.4%)and blaIMP (45.9%) genea were detected genes .
conclusion : The presence of ompA and blaIMP genes in acinetobacter baumannii means it can resist antibiotic and cause severe infactions. Detection these genes early helps improve treatment and prevent spread .
