This study aimed to examine the dissemination of extended spectrum B-lactamase (ESBL) producing isolates of Klebsiella pneumoniae obtained from patients with urinary tract infection who admitted Al-Diwaniya Teaching Hospital from December 2013 to April 2014. A total of 85 urine samples collected, after the first culturing thirty isolates of Klebsiella pneumoniae were obtained. The results of primary screening showed that all isolates were found to be resistance to B- actamase antibiotics (ampicillin and amoxicillin) at percentage (100%). All isolates were screened for their antibiotic resistance against 8 antibiotics of different classes using Kirby-Bauer disk diffusion method. The results showed that all isolates were resistance to ceftazidime, ceftriaxone, and cefepime in (100%), while the antibiotics tetracycline and levofloxacin (66.6%) of isolates were resistance. Half of isolates were resistance against ciprofloxacin and tobramycin, all isolates were sensitive to gentamycin in percentage (100%). Present study revealed increase the percentage of ESBL producing Klebsiella pneumoniae 11 isolates (36.7%) in compared with other studies, also the isolates were resistance to third and fourth generation of cephalosporine.
