Background ;This study was carried out on (100) patients (64 females & 36 males) with different severity of acne vulgaris with an age range(11-40 )year, seen in AL-Diwaniya Teaching Hospital/ Department of Dermatology, from December 2011 to February 2012, another (50) apparently healthy subjects were taken as a control group. Materials; Blood samples were collected from both groups, genomic DNA was extracted from peripheral blood leukocytes for further molecular study, polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique was used .
Results; genotyping of TNF-α revealed three genotypes; the wild homozygous GG type, the heterozygous GA & the mutant homozygous AA type, the genotype frequency for these three types in acne patients was (29%, 67% & 4%) respectively, in control group, however, it was (58%, 30% & 12%) respectively. The frequency for GA type was found statistically significantly increased in acne patients compared to the healthy controls (P<0.001), significant association was found between the minor A allele and females patients(40.6%) compared to healthy females(28.8%) ,P value 0.001,also all the female that had severe and very severe acne carry the GA genotype.
