The Role of Protamine 1 Gene Polymorphism and Anti FSH Antibody in Infertile Couples

Abstract

Background: Infertility is a worldwide problem which affects approximately 15% of all couples, and is defined as, inability to conceive after twelve months of contraceptive-free unprotected intercourse. Materials and methods: Optical density of anti follicle stimulation hormone antibody in the serum was detected by enzyme–linked immunosorbent assay technique and from which anti follicle stimulation hormone antibody concentrations were evaluated according to cut-off. One polymorphic sites of Protamine 1 Gene was genotyped on 72 couples suffering from infertility. Another group consist of 20 couples apparently healthy individuals. Genotypes were determined by the polymerase chain restriction fragment length polymorphism (PCR-RFLP) method. Results: Anti FSH antibody result demonstrated differences in antibody values between the patients and the control group with significant statistically, the frequency of the reported SNP was no significantly different compared to normal fertile males, which suggest that such SNP may not serve as a good molecular marker for genetic diagnosis of male infertility. Conclusion: The SNP G197T are completely absent and are not associated with male infertility with aforementioned, therefore these SNPs may not represent as a molecular marker for the diagnosis of genetic cause of male infertility in our studied population.