The results of our study showed that percentage of bacterial contamination in operating rooms was (66.2%) before disinfection method, and after disinfection method was (47.5%). High contamination percentage (100%) was in room floor covers and was decreased to (60%) after disinfectant. The lowest percentage of contamination (1%) was in surgical instruments and disappeared after disinfectant. The percentage of contamination in disinfectants and antiseptics was (55%), high contamination percentage (75%) was in Chloroxylenol (Dettol) whereas the owest (25%) was in Formalin.