Evaluation of A Disintegrin and Metalloprotein33 Gene Polymorphism in Bronchial Asthma

Abstract

Background: A disintegrin and metalloprotein 33 (ADAM33) gene is the first asthma candidate gene identified by positional cloning, may be associated with lung function decline and bronchial hyperresponsiveness. However, replication results have been inconclusive in smaller previous study populations probably due to inconsistence in asthma phenotypes or yet unknown environmental influences. This study aimed to further elucidate the role of ADAM33 polymorphisms (SNPs) in a genetic analysis of our case- control. Materials and methods: One polymorphic sites (V4) of ADAM33 gene was genotyped in 69 patients with bronchial asthma, and 20 healthy controls. Genotypes were determined by the polymerase chain restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using the chi-square test and SPSS version 20 computer software (Statistical Package for Social Sciences) in association with Microsoft Excel 2010. Results: The single nucleotide polymorphisms V4 G/C, of the ADAM33 gene may be participate in the susceptibility of bronchial asthma in the Iraqi population. Conclusion: Although ADAM33-V4 polymorphism not associated with asthma in many population, our study confirmed significant correlation between ADAM33-V4 and asthma .