Detection of Serotype Gene of Klebsiella Pneumoniae Isolated from Different Clinical Cases of Hospitalized Infections in Al-Diwaniya city

Abstract

This study aimed to identify serotype of Klebsiella Pneumonia  isolated from different clinical cases of hospitalized infections( urinary tract infection, respiratory infection, burns & wounds) by using polymerase chain reaction(PCR).A total of 110 clinical specimens from in-patients with nosocomial infections(including 47 respiratory infection,37  UTI , burns  & wound infections 26) in Al-Diwaniya Teaching Hospital during the period from December 2012 –March 2013 were collected. K. Pneumonia  isolated from 35.4% of the specimens distributed as 18.18% sputum specimens,10.90% urine specimens & 6.36% burns & wounds specimens.

K1,K2,K5,K20,K54 & K57 serotypes of K. Pneumonia  were successfully detected in multiplex PCR.The results revealed that 28 specimens(25.4%) were type able including 7 positive K1(Amplicon size 1046bp) serotype isolates ( 4 from sputum,2 from urine & 1 from burn), 6 positive of K2(Amplicon size1121) serotype isolates (3 from urine,2 from sputum & 1 from wound),3 positive for K5(Amplicon size 999bp) serotype isolates( 2 from sputum & 1 from urine),2 positive for K20(Amplicon size 1116bp) serotype isolates from urine 2 positive of K54(Amplicon size 881bp) serotype isolates were one from sputum & the other from urine& finally 8 positive of K57(Amplicon size 1182bp) serotype isolates(4 from sputum,2 from urine & 2 from burns & wounds).

However, among 11 (10%) specimens, K. Pneumonia  were non type able.

This study revealed that there were predominance of K1,K2 & K57 serotype isolates in sputum, urine burns & wounds of patients with nosocomial infections.