Detection and determination of hepatitis B using molecular and serological methods in patients with hepatitis B in Al-Diwaniya Iraq

Mohsin Abdulkhudhur Al-Zubaidi; Masoumeh Bahreini; Mohammad Reza Sharif moghadam; Wissam Saleh Abood Al-Rubaye

doi:10.28922/qmj.2019.15.1.87-97

Authors

Mohsin Abdulkhudhur Al-Zubaidi General Teaching Hospital,Dewaniyah-IRAQ
Masoumeh Bahreini Assistant Professor Microbiology - University of Ferdowsi - Faculty of Science - Iran
Mohammad Reza Sharif moghadam Assistant Professor Microbiology and Molecular Biology - University of Ferdowsi - Faculty of Science - Iran
Wissam Saleh Abood Al-Rubaye Assistant Professor Microbiology (medical viruses) - University of Al-Qadisiyah - College of Medicine – Iraq

DOI:

https://doi.org/10.28922/qmj.2019.15.1.87-97

Keywords:

Hepatitis B, HBs Ag, Anti HBs, Anti HBe, HBV DNA, Diwaniya – Iraq

Abstract

Hepatitis B virus (HBV) is one of the most prevalent pathogens in the world and infection with this virus is a serious threat for public health. The pathogenesis of HBV depends on the critical interplay between viral and host factors.

Test 88 samples for the detection of serological markers using ELISA and PCR technique for positive HBsAg. Regarding population group (HBsAg) positive, the seroprevalence rate of HBsAg, anti-HBs, anti-HBc IgM, HBc Total HBeAg , anti-HBe and HBV DNA were 25%, 5%, 12%, 10%, 9%, 17% and 22% respectively. In this study, hepatitis was diagnosed with the following tests HBsAg, anti-HBs, anti-HBc IgM, anti-HBc total using the ELISA device. HBV DNA was also tested using PCR. To determine the stage of the disease, the HBeAg, anti-HBe and ALT test was added and the stages of the disease were Chronic HBV, Acute HBV, Incubation period, Recovery stage, Window stage, false positive and carrier stage 36.36%, 25%, 17.07%, 15.90%, 2.27%, 2.27 % and 1.13% respectively.