The Molecular Identification of agg and hyl genes of Enterococcus faecalis isolated from different clinical sampl
Molecular Detection of agg and hyl genes in Enterococcus faecalis isolates
DOI:
https://doi.org/10.28922/qmj.2023.19.2.65-68Keywords:
Enterococcus faecalis, virulence factors, PCR technique.Abstract
Background: Enterococcus faecalis was gram-positive, facultative anaerobic, catalase-negative, oxidase negative, non-spore forming bacteria belonging to the genus Enterococcus frequently live in the intestines of healthy people. It typically appear in pairs or chains of various lengths. And its Microorganisms with mesophilic characteristics that grow for (ten – fifty four)?C and optimal temp comprised between 30?C and 35?C . Also, they are able to grow in a huge range of pH from 4.4 and 9.6 and in hyper salty media with 6.5% NaCl. Objective: This study aimed to molecular detection of the some genes responsible for virulence factor (agg and hyl) by PCR technique. Materials and Methods: 130 clinical specimens acquired from patients were who admitted to Feminine and Children Teaching Hospital in Al-Diwaniyah city of during a period extending from (November 2022 to march 2023). Each specimen tested for culture on multiple types of media (Blood agar, MacConkey, Bile Esculin agar and chromogenic ager) followed by biochemical testing (Catalase test, Oxidase test). After cultivation the samples were identified by using the VITEK-2 compact system. The isolates were investigate genotypically for harboring virulence factors genes that include agg and hyl by molecular methods PCR. Results: VITEK-2 compact system revealed that only12 isolates were identified as Enterococcus faecalis. The results showed the percentage of genes were (75%) and (41.6%) for agg and hyl respectively. Coclusion: It has been found that the existence of several virulent factor genes in E. faecalis, such as agg and hyl, boosts this pathogen's pathogenicity and amongst E. faecalis isolates, the agg gene exhibited a greater proportion.
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