Gene Expression of Biofilm Regulatory Protein A (Brp A) in Streptococcus mutans Isolated from Oral Cavity

Abstract

A total of 100 oral swabs were collected from patients  suffering from dental caries and priodontal infections, ranging from 15-65 years old of both genders whom admitted to the Al-Diwaniyah Teaching Special Center of Dentistry within the period from December 2012 to March 2013, in order to  quantify the gene expression of biofim regulatory protein (Brp A) in Streptococcus mutans (S. mutans) isolates by using the Real Time polymerase chain reaction. The gene expression of biofilm regulatory protein A(Brp A) was quantified in comparison with housekeeping gene 16s rRNA as a reference gene of biofilm S.mutans by using reverse transcriptase polymerase chain reaction (RT-PCR).The results revealed that the expression (absolute) of BrpA gene is decreased in the test treatment (S.mutans with 1% glucose+ 50% curcumine) in comparison with control  (S.mutans grown in 1% glucose only). In conclusion, the present study established the role of Brp A gene in biofilm formation and recommended to possibility of using the curcumine  as adddative material in composition of toothpaste due to their inhibitory and reduction effect on biofilm S.mutans isolates.