The use of Omp W Gene in Detection of Vibrio cholerae Isolated from Diarrhea Cases of Children in AL-Diwaniya Province

Abstract

A total of 221 stool samples were collected from children suffering from watery diarrhea, less than 15 years old of both genders whom admitted to the Maternity and Children Teaching Hospital in Al- Diwaniya Province, In addition to that, 27 water samples were also collected from three different loci of ALDiwaniya River, at the period of October 2008 to June 2009,in order to evaluate the routine laboratory diagnostic procedures in the diagnosis the multi-serogroups or serotype of Vibrio cholerae strains and compared them with molecular technique as Polymerase chain reaction (PCR).
Vibrio cholerae has been isolated and identified by using culturing method in addition to biochemical tests, API 20E diagnostic kit. Serotyping by using polyvalent Vibrio cholerae, O1antisera, and monovalent Ogawa and Inaba revealed that the most of clinical V. cholerae were of serogroup O1, while all the V. cholerae isolated from surface water of AL- Diwanyia river was Non-O1 serogroup. PCR technique was used to detect ompW gene encoding to outer membrane protein of V. cholerae. Based on the PCR results, the rate of Vibrio cholerae isolation from stool samples was 5.9%. PCR results showed that there were high specificity (100%, 100%, 97% and 86%) in detection of Vibrio cholerae strains versus each of cultural, biochemical, API 20E system and serological tests, respectively.