Determination of Antimicrobial Activity of Bi2O3 nanospheres Against Multi-Drug Resistant Pathogenic Bacteria
DOI:
https://doi.org/10.28922/qmj.2018.14.25.86-94الكلمات المفتاحية:
Bi2O3nanospheres، Antimicrobial Activity، well diffusion method، S. aureus, E.coliandKlebsiella sp.الملخص
The main objective of this study was to evaluate the bactericidal activity of Bi2O3nanospheres against the antimicrobial resistant isolates included Staphylococcus aureus, Escherichia coliandKlebsiella sp.Bismuth Oxide Nanospere particles was prepared by chemical reduction technique with (11-30)nm average diameter, the colloidal of Bi2O3nanospheres was characterized by using X-ray diffraction (XRD), TransmitionElectron Microscopy (TEM) and UV–Vis spectrophotometry. Three pathogenic strains of multi-drug resistant bacteria of each Staphylococcus aureus, Escherichia coliandKlebsiella sp.were isolated and identified from different clinical samples usingtraditional methods on bacteriological culture media. The antibacterial susceptibility of (Amoxicillin, Cefotaxime,Gentamicin, and Ciproflouxacin) were analyzed on Muller Hinton agar plate and the activity of Bi2O3nanospheres was assayed using well diffusion method against tested bacteria. The results of antibacterial susceptibility of bacterial isolates exhibited high resistance to the antibiotics that used in the current study. According to these findings thecolloidalof Bi2O3nanospheres revealed highest activity when using 100 ?g/ml and the mean of inhibition zone was 18-22 mm for each S. aureus, E. coliandKlebsiella sp. However, the study showed slight effects (meanof inhibition zones ? 13 mm) when used50?g/ml of Bi2O3nanospheres. In same way, the E. coliandKlebsiellasp were more susceptible (mean of inhibition zones;15-12mm) for 150?g/mlofBi2O3nanospheres, than S. aureus. It is concluded that theBi2O3nanosphereshave insitu effectiveness an displayed a good antibacterial action against the tested bacterial isolates.