Determination of Antimicrobial Activity of Bi2O3 nanospheres Against Multi-Drug Resistant Pathogenic Bacteria

Abeer A. Alredha; Wasna'a M. Abdulridha; Zuhair S. Alsehlawi; Ahmed N. Abd

doi:10.28922/qmj.2018.14.25.86-94

Authors

Abeer A. Alredha Department of Basic Science, College of Dentistry, Kufa University, Iraq
Wasna'a M. Abdulridha Department of Basic Science, College of Dentistry, Kufa University, Iraq
Zuhair S. Alsehlawi Head of Department of Basic Science, College of Dentistry, Kufa University, Iraq
Ahmed N. Abd Physics Department, Science Faculty, Al-Mustansiriyah University, Iraq

DOI:

https://doi.org/10.28922/qmj.2018.14.25.86-94

Keywords:

Bi2O3nanospheres, Antimicrobial Activity, well diffusion method, S. aureus, E.coliandKlebsiella sp.

Abstract

The main objective of this study was to evaluate the bactericidal activity of Bi₂O₃nanospheres against the antimicrobial resistant isolates included Staphylococcus aureus, Escherichia coliandKlebsiella sp.Bismuth Oxide Nanospere particles was prepared by chemical reduction technique with (11-30)nm average diameter, the colloidal of Bi₂O₃nanospheres was characterized by using X-ray diffraction (XRD), TransmitionElectron Microscopy (TEM) and UV–Vis spectrophotometry. Three pathogenic strains of multi-drug resistant bacteria of each Staphylococcus aureus, Escherichia coliandKlebsiella sp.were isolated and identified from different clinical samples usingtraditional methods on bacteriological culture media. The antibacterial susceptibility of (Amoxicillin, Cefotaxime,Gentamicin, and Ciproflouxacin) were analyzed on Muller Hinton agar plate and the activity of Bi₂O₃nanospheres was assayed using well diffusion method against tested bacteria. The results of antibacterial susceptibility of bacterial isolates exhibited high resistance to the antibiotics that used in the current study. According to these findings thecolloidalof Bi₂O₃nanospheres revealed highest activity when using 100 ?g/ml and the mean of inhibition zone was 18-22 mm for each S. aureus, E. coliandKlebsiella sp. However, the study showed slight effects (meanof inhibition zones ? 13 mm) when used50?g/ml of Bi₂O₃nanospheres. In same way, the E. coliandKlebsiellasp were more susceptible (mean of inhibition zones;15-12mm) for 150?g/mlofBi₂O₃nanospheres, than S. aureus. It is concluded that theBi₂O₃nanosphereshave insitu effectiveness an displayed a good antibacterial action against the tested bacterial isolates.